In the application of membrane separation technology, microporous membrane is the most widely used type of membrane, which is simple and quick to use. It has the characteristics of large gas flux, high particle retention rate, good temperature resistance, resistance to strong acids, alkalis, organic solvents and oxidants, aging resistance, non-stick, non-flammable, and non-toxic, and good biocompatibility. Its materials are divided into two categories: organic and inorganic. In view of the separation characteristics of microporous membranes, the application scope of microporous membranes is mainly to intercept particles, bacteria, and other pollutants from the gas phase and liquid phase to achieve purification, separation, and purpose of concentration. For microfiltration, the retention characteristics of the membrane are expressed by the pore size of the membrane, usually in the range of 0.1-1um, so the microporous filtration membrane can separate large-diameter bacteria and suspended solids. It can be used for clarification and air sterilization of general liquids. It is widely used in scientific research, medicine and health, biochemistry, food, energy, the microelectronics industry, nanotechnology, environmental protection, and other departments.
1. Application of microporous membrane in chromatographic analysis
Microporous membranes and filters are widely used in chemical analysis research. Analytical instruments such as High-Performance Liquid Chromatography (HPLC), Gas Chromatography (GC), Gel Chromatography (GPC), Ion Chromatography (IC), and Inductively Coupled Plasma Mass Spectrometry (ICP-MS) all require relevant The filter product to remove particulate impurities in the sample, prevents the blockage or damage of key components such as pipelines, pumps or separation columns, prolongs the service life of the whole machine, and ensures the accuracy of the analysis results.
Taking GPLC as an example, a 0.22 μm or 0.45 μm needle filter needs to be used before injection, and the sample is manually injected with a syringe to filter the sample to remove insoluble particles. The mobile phase of HPLC also needs to use a 0.22μm or 0.45μm microporous filter with a glass suction filter bottle for vacuum filtration to remove particles before use, or an online filtration/degassing device can also be installed on the HPLC. Mobile phase filtration removes particles and at the same time removes microscopic bubbles dispersed in them, reducing baseline fluctuations.
2. Application of microporous membrane in liquid clarification/sterilization/mycoplasma removal
In the operation of biochemical experiments, various solutions are often required to be clarified. The most common treatment method is microporous membrane filtration.
For solutions with high particle content or viscosity, in order to reduce the frequent replacement of filter media due to clogging, ultra-fine glass fiber paper with high dirt holding capacity and low resistance can be selected for filtration, but it should be noted that downstream fibers may fall off. For finer particles with smaller sizes, a microfiltration membrane with higher precision is required to achieve removal. In the process of use, the appropriate material should be selected for filtration according to the characteristics of the solution (pH, polarity, solubility parameters, temperature, etc.). Microporous membranes and filters can be used for the sterile filtration of solutions after being sterilized by autoclaving, gamma irradiation, or EO fumigation, especially those containing temperature-sensitive substances that cannot be sterilized by high-temperature steam. solution. Therefore, this method of sterilization is also called "cold sterilization".
Common media, buffers, serum, protein solutions, etc. in biochemical experiments can be sterilized by microporous filters. Microporous filters that pass the 10^7 CFU/cm² challenge level test for B. deficient are considered "sterile grade", with a pore size class defined as 0.22μm or 0.2μm. Microporous membranes with a pore size grade of 0.1 μm from the outside are considered to be able to remove mycoplasma to a certain extent.
The material of the microporous filter membrane
Mainly: nylon 66 (Nylon66), nylon 6 (Nylon6), polyethersulfone (PES), mixed cellulose (MCE), polypropylene (PP), polytetrafluoroethylene (PTFE), polyvinylidene fluoride (PVDF).
Wuxi Lenge Purification Equipments Co., Ltd. is a professional manufacturer of purification equipment, we have a variety of microporous membranes for you to choose from. Suitable for pharmaceuticals, laboratories, food and beverage, electronics, etc. We also have stainless steel furniture. If you are interested in purification equipment and stainless steel furniture, please contact us! You can contact us at 86-510-85189266. Or send an email to firstname.lastname@example.org.
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